ChIP-seq
ChIP
Fix the cells to cross link the protein to the DNA
Then immunoprecipitate the target protein out with an antibody
Then you can do sequencing or PCR
History
H3 pan as loading control for checking histone modifications
Proximity Ligation-Assisted ChIP-seq (PLAC-seq)
Proximity ligation is conducted in nuclei prior to chromatin shearing and immunoprecipitation
PLAC-seq requires less input materials, and has better accuracy than ChIA-PET
Backlinks
- chip-seq "mock" and "control"
- ChIP Sequencing Overview
- Peak calling with MACS2 | Introduction to ChIP-Seq using high-performance computing
- The History of the Biggest Technique of 2009 - Who invented ChIP-Seq? | The Snarky Scientist
- Formaldehyde Crosslinking: A Tool for the Study of Chromatin Complexes* - Journal of Biological Chemistry
- Malladi_2020: Total functional score of enhancer elements identifies lineage-specific enhancers that drive differentiation of pancreatic cells
- Mikkelsen_2007: Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
- BIOL5420 - Molecular Biology
- BIOL5449 - Cell Biology
- Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET)