ATAC-seq
Came out in 2013
Direct measure of how open a piece of DNA is
This a proxy to how easily a transcription factor can bind to the genome
Uses TN5 transposase, which binds to open chromatin and inserts DNA sequencing adapters
Libraries are then created and sequenced
scATAC-seq
Backlinks
- 2022 Functional Genomics Lab Notebook
- Single-cell epigenomics reveals mechanisms of human cortical development : (Ziffra_2021)
- ATAC-Seq, scATAC-Seq and Chromatin Dynamics in Single-Cells (Jason Buenrostro) - Epigenetics Podcast
- Dr. Jason Buenrostro -- an Inventor of ATAC-Seq -- Discusses the Utility of Single-Cell ATAC-Seq - YouTube
- Dr. Jason Buenrostro Discusses How ATAC-Seq Works - YouTube
- Malladi_2020: Total functional score of enhancer elements identifies lineage-specific enhancers that drive differentiation of pancreatic cells