CRISPR
Clustered Regularly Interspaced Short Palindromic Repeats
Class II CRISPR systems
Cas9
DNA/RNA targeting
Case12a
DNA targeting
Cas13a
RNA targeting
Targeted mutagenesis using CRISPR system
Limitations or challenges
Cas9 is a large protein
May have a potential existing immunity
Small amount of available vectors
Could be fixed by CRISPR-Casϕ @Pausch2020
Off target edits can occur
Backlinks
- March 10, 2022
- Nobel Prize in Chemistry 2020
- BioRender | Nobel Prize in Chemistry 2020
- Sternberg_2015: Conformational control of dna target cleavage by crispr--cas9
- Corsi_2015: A transparent window into biology: a primer on caenorhabditis elegans
- BIOL5420 - Molecular Biology
- BIOL6193 - Colloquium in Molecular and Cell Biology
- Journal Club
- CRISPR